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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in many tumors, which leads to chromosome instability, and thus might add to tumorigenesis. This protein was located to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important job in marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have already been explained. Two further splice variants ended up described but comprehensive nucleotide sequence information is not really however readily available.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide ordinarily is made up of the epitope identified by the antibody.
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This guide outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This guidebook outlines even more achievable optimization of mobile and immunoassay parameters to guarantee the very best success are...
Antibodies sure to the blocking peptide no more bind towards the epitope on the goal protein. By comparing the staining through the blocked antibody as opposed to the antibody by yourself, one can see which staining is specific.
Focus on Information and facts Cyclin E belongs to the highly conserved cyclin family members, whose customers are characterised by a extraordinary periodicity in protein abundance through the cell cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation styles which add to the temporal coordination of each and every mitotic celebration. Cyclin E kinds a fancy with and features as a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates within the G1-S phase boundary and it is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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